Fluoroquinolone resistance in Campylobacter jejuni and Campylobacter coli from poultry and human samples assessed by PCR-restriction fragment length polymorphism assay
dc.contributor.author | Sierra Arguello, Yuli Melisa | pt_BR |
dc.contributor.author | Furian, Thales Quedi | pt_BR |
dc.contributor.author | Perdoncini, Gustavo | pt_BR |
dc.contributor.author | Moraes, Hamilton Luiz de Souza | pt_BR |
dc.contributor.author | Salle, Carlos Tadeu Pippi | pt_BR |
dc.contributor.author | Rodrigues, Laura Beatriz | pt_BR |
dc.contributor.author | Santos, Luciana Ruschel dos | pt_BR |
dc.contributor.author | Gomes, Marcos José Pereira | pt_BR |
dc.contributor.author | Nascimento, Vladimir Pinheiro do | pt_BR |
dc.date.accessioned | 2018-09-27T02:34:37Z | pt_BR |
dc.date.issued | 2018 | pt_BR |
dc.identifier.issn | 1932-6203 | pt_BR |
dc.identifier.uri | http://hdl.handle.net/10183/182852 | pt_BR |
dc.description.abstract | The objective of this study was to determine fluoroquinolone resistance in Campylobacter spp from poultry and human isolates. Forty-one Campylobacter jejuni isolates (30 of poultry origin and 11 of human origin) and 11 Campylobacter coli isolates (10 of human origin and 1 of poultry origin) were examined for ciprofloxacin, norfloxacin, and nalidixic acid resistance using the minimal inhibitory concentration (MIC) method. Thereafter, the isolates were analyzed by PCR±Restriction Fragment Length Polymorphism (RFLP) assay for detection of Thr-86 mutation. Finally, DNA sequencing was performed for confirmation of gyrA gene mutation. A complete correlation was observed between MICs, PCR-RFLP assay, and sequencing. The results revealed high quinolone resistance rates for C. jejuni (100%) and C. coli (100%) isolates obtained from poultry and moderate resistance for C. jejuni (9.1%) and C. coli (40%) samples of human origin. A mutation in codon 86 of the gyrA gene with a Thr-to-Ile substitution is reported to be the main cause of high resistance to quinolones. This mutation can be analyzed by PCR-RFLP assay, which has been proven to be a simple and fast method for the detection of fluoroquinolone resistance in Campylobacter spp. | en |
dc.format.mimetype | application/pdf | pt_BR |
dc.language.iso | eng | pt_BR |
dc.relation.ispartof | PLOS ONE Journal. San Francisco, CA. Vol. 13, no. 7 (July 2018), e0199974, 9 p. | pt_BR |
dc.rights | Open Access | en |
dc.subject | Resistência antimicrobiana | pt_BR |
dc.subject | Fluoroquinolonas | pt_BR |
dc.subject | Campylobacter jejuni | pt_BR |
dc.subject | Campylobacter coli | pt_BR |
dc.subject | Aves | pt_BR |
dc.subject | Seres humanos | pt_BR |
dc.subject | Polimorfismo de fragmento de restrição | pt_BR |
dc.subject | Análise de sequência de DNA | pt_BR |
dc.title | Fluoroquinolone resistance in Campylobacter jejuni and Campylobacter coli from poultry and human samples assessed by PCR-restriction fragment length polymorphism assay | pt_BR |
dc.type | Artigo de periódico | pt_BR |
dc.identifier.nrb | 001075036 | pt_BR |
dc.type.origin | Estrangeiro | pt_BR |
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