Effect of blood contamination on the push-out bond strength of calcium silicate cements

Abstract

ARTICLE Effect of Blood Contamination on The Push-Out Bond Strength of Calcium Silicate Cements Flavia Kolling Marquezan1 Patricia Maria Poli Kopper1 Angela Isabel dos Santos Dullius2 Diego Machado Ardenghi3 Renata Grazziotin-Soares3 1Department of Conservative Dentistry, UFRGS - Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil 2Department of Statistics, UFSM - Universidade Federal de Santa Maria, Santa Maria, RS, Brazil 3College of Dentistry, University of Saskatchewan, SK, Canada ABSTRACT This study investigated the effect of blood-contamination on the push-out bond strength of BiodentineTM (BD) and MTA Angelus® (MTA-A) to root dentin over time. Twenty-five teeth were sectioned horizontally to obtain 120 root slices. The lumens were filled with MTA-A or BD: 60 for each cement (30 uncontaminated and 30 blood contaminated). Push out bond strength to dentin was assessed at 24 h (n=10), 7 days (n=10) and 28 days (n=10). Failure modes were classified as: cohesive, adhesive or mixed failure. Two-way ANOVA was used to investigate the interaction between blood contamination vs. hydration period. Mann Whitney test compared different materials in each period, and it also compared the contaminated versus uncontaminated material for each period. Friedman, followed by Dunn`s test, compared periods of hydration for each material, regardless of blood contamination. Failure modes were reported descriptively. The interaction hydration period vs. blood contamination was highly significant for MTA-A (P=0.001) and it was not significant for BD (P=0.474). There were no differences between bond strength of uncontaminated and contaminated BD in any of the periods. Bond strength of uncontaminated MTA-A increased at each time of hydration; but it remained stable over time for blood-contaminated samples. BD had higher bond strength than MTA-A in all periods of hydration. Cohesive failure predomi