Quillaja brasiliensis saponin-based nanoparticulate adjuvants are capable of triggering early immune responses
dc.contributor.author | Cibulski, Samuel Paulo | pt_BR |
dc.contributor.author | Rivera Patron, Mariana | pt_BR |
dc.contributor.author | Ettlin, Gustavo Mourglia | pt_BR |
dc.contributor.author | Casaravilla, Cecilia | pt_BR |
dc.contributor.author | Yendo, Anna Carolina | pt_BR |
dc.contributor.author | Fett Neto, Arthur Germano | pt_BR |
dc.contributor.author | Chabalgoity, José Alejandro | pt_BR |
dc.contributor.author | Moreno, María | pt_BR |
dc.contributor.author | Roehe, Paulo Michel | pt_BR |
dc.contributor.author | Silveira, Fernando | pt_BR |
dc.date.accessioned | 2019-01-19T02:33:41Z | pt_BR |
dc.date.issued | 2018 | pt_BR |
dc.identifier.issn | 2045-2322 | pt_BR |
dc.identifier.uri | http://hdl.handle.net/10183/187937 | pt_BR |
dc.description.abstract | Commercially available saponins are extracted from Quillaja saponaria barks, being Quil A® the most widely used. Nanoparticulate immunostimulating complexes (ISCOMs or ISCOMATRIX) formulated with these, are able to stimulate strong humoral and cellular immune responses. Recently, we formulated novel ISCOMs replacing QuilA® by QB-90 (IQB-90), a Quillaja brasiliensis leaf-extracted saponin fraction, and reported that IQB-90 improved antigen uptake, and induced systemic and mucosal antibody production, and T-cell responses. However, its mechanism of action remains unclear. In this study we provide a deeper insight into the immune stimulatory properties of QB-90 and ISCOMATRIX-like based on this fraction (IMXQB-90). We show herein that, when used as a viral vaccine adjuvant, QB-90 promotes an “immunocompetent environment”. In addition, QB-90 and IMXQB-90 induce immune-cells recruitment at draining-lymph nodes and spleen. Subsequently, we prove that QB-90 or IMXQB-90 stimulated dendritic cells secret IL-1β by mechanisms involving Caspase-1/11 and MyD88 pathways, implying canonical inflammasome activation. Finally, both formulations induce a change in the expression of cytokines and chemokines coding genes, many of which are up-regulated. Findings reported here provide important insights into the molecular and cellular mechanisms underlying the adjuvant activity of Q. brasiliensis leaf-saponins and its respective nanoparticles. | en |
dc.format.mimetype | application/pdf | pt_BR |
dc.language.iso | eng | pt_BR |
dc.relation.ispartof | Scientific reports. London. Vol. 8, article 13582, p. 1-15 | pt_BR |
dc.rights | Open Access | en |
dc.subject | Quillaja brasiliensis : Saponinas | pt_BR |
dc.subject | Immunostimulating complexes (ISCOMs) | en |
dc.subject | Resposta imune | pt_BR |
dc.subject | Myeloid differentiation primary response (MyD88) | en |
dc.subject | Immunocompetent environment | en |
dc.subject | Saponin fraction | en |
dc.subject | Inflammasome activation | en |
dc.title | Quillaja brasiliensis saponin-based nanoparticulate adjuvants are capable of triggering early immune responses | pt_BR |
dc.type | Artigo de periódico | pt_BR |
dc.identifier.nrb | 001082740 | pt_BR |
dc.type.origin | Estrangeiro | pt_BR |
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