Reduced cortical renal GLUT1 expression induced by angiotensin-converting enzyme inhibition in diabetic spontaneously hypertensive rats
dc.contributor.author | Souza, M. S. | pt_BR |
dc.contributor.author | Machado, Ubiratan Fabres | pt_BR |
dc.contributor.author | Okamoto, Maristela Mitiko | pt_BR |
dc.contributor.author | Bertoluci, Marcello Casaccia | pt_BR |
dc.contributor.author | Ponpermeyer, C. | pt_BR |
dc.contributor.author | Leguisamo, Natalia Motta | pt_BR |
dc.contributor.author | Azambuja, Felipe | pt_BR |
dc.contributor.author | Irigoyen, Maria Claudia Costa | pt_BR |
dc.contributor.author | Schaan, Beatriz D'Agord | pt_BR |
dc.date.accessioned | 2010-04-24T04:15:52Z | pt_BR |
dc.date.issued | 2008 | pt_BR |
dc.identifier.issn | 0100-879X | pt_BR |
dc.identifier.uri | http://hdl.handle.net/10183/21227 | pt_BR |
dc.description.abstract | Diabetes in spontaneously hypertensive rats is associated with cortical renal GLUT1 and GLUT2 overexpression. Our objective was to evaluate the effect of the angiotensin-converting enzyme blockade on cortical renal GLUT1 and GLUT2 expression, urinary albumin and urinary TGF-β1. Streptozotocin, 50 mg/kg, or citrate buffer (N = 16) was administered as a single injection into the tail vein in adult spontaneously hypertensive rats (~260 g). Thirty days later, these diabetic spontaneously hypertensive rats received ramipril by gavage: 0.01 mg·kg-1·day-1 (D0.01, N = 14), 1 mg·kg-1·day-1 (D1, N = 9) or water (D, N = 11) for 15 days. Albumin and TGF-β1 (24-h urine), direct arterial pressure, renal tissue angiotensin-converting enzyme activity (fluorometric assay), and GLUT1 and GLUT2 protein levels (Western blot, renal cortex) were determined. Glycemia and glycosuria were higher (P < 0.05) in the diabetic rats compared with controls, but similar between the diabetic groups. Diabetes in spontaneously hypertensive rats lowered renal tissue angiotensin-converting enzyme activity (40%), which was reduced further when higher ramipril doses were used. Diabetes associated with hypertension raised GLUT1 by 28% (P < 0.0001) and GLUT2 by 76% (P = 0.01), and both doses of ramipril equally reduced cortical GLUT1 (D vs D1 and vs D0.01, P ≤ 0.001). GLUT2 levels were reduced in D0.01 (P < 0.05 vs D). Diabetes increased urinary albumin and TGF-β1 urinary excretion, but the 15-day ramipril treatment (with either dose) did not reduce them. In conclusion, ramipril is effective in lowering renal tissue angiotensin-converting enzyme activity, as well as blocking cortical GLUT1 overexpression, which may be beneficial in arresting the development of diabetic nephropathy. | en |
dc.format.mimetype | application/pdf | |
dc.language.iso | eng | pt_BR |
dc.relation.ispartof | Brazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas. RiBeirão Preto, SP. Vol. 41, n. 11 (Nov. 2008), p. 960-968 | pt_BR |
dc.rights | Open Access | en |
dc.subject | Angiotensin-converting enzyme inhibition | en |
dc.subject | Inibidores da enzima conversora da angiotensina | pt_BR |
dc.subject | Diabetic nephropathy | en |
dc.subject | Diabetes mellitus | pt_BR |
dc.subject | Hipertensão | pt_BR |
dc.subject | Glucose transporter proteins | en |
dc.subject | Hypertension | en |
dc.subject | Streptozotocin | en |
dc.title | Reduced cortical renal GLUT1 expression induced by angiotensin-converting enzyme inhibition in diabetic spontaneously hypertensive rats | pt_BR |
dc.type | Artigo de periódico | pt_BR |
dc.identifier.nrb | 000673195 | pt_BR |
dc.type.origin | Nacional | pt_BR |
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