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dc.contributor.authorMichels, Moniquept_BR
dc.contributor.authorAbatti, Mariane R.pt_BR
dc.contributor.authorÁvila, Pricila Romão Marcondespt_BR
dc.contributor.authorVieira, Andriele Aparecida da Silvapt_BR
dc.contributor.authorBorges, Heloísa de Medeirospt_BR
dc.contributor.authorCarvalho Junior, Celso Carneiropt_BR
dc.contributor.authorWendhausen, Diogo Luizpt_BR
dc.contributor.authorGasparotto, Jucianopt_BR
dc.contributor.authorRibeiro, Camila Tiefenseept_BR
dc.contributor.authorMoreira, Jose Claudio Fonsecapt_BR
dc.contributor.authorGelain, Daniel Penspt_BR
dc.contributor.authorPizzol, Felipe Dalpt_BR
dc.date.accessioned2020-11-20T04:16:09Zpt_BR
dc.date.issued2020pt_BR
dc.identifier.issn1582-1838pt_BR
dc.identifier.urihttp://hdl.handle.net/10183/215330pt_BR
dc.description.abstractWe aim to characterize the kinetics of early and late microglial phenotypes after systemic inflammation in an animal model of severe sepsis and the effects of minocycline on these phenotypes. Rats were subjected to CLP, and some animals were treated with minocycline (10 ug/kg) by i.c.v. administration. Animals were killed 24 hours, 5, 10 and 30 days after sepsis induction, and serum and hippocampus were collected for subsequent analyses. Real-time PCR was performed for M1 and M2 markers. TNF-α, IL-1β, IL-6, IL-10, CCL-22 and nitrite/nitrate levels were measured. Immunofluorescence for IBA-1, CD11b and arginase was also performed. We demonstrated that early after sepsis, there was a preponderant up-regulation of M1 markers, and this was not switched to M2 phenotype markers later on. We found that up-regulation of both M1 and M2 markers co-existed up to 30 days after sepsis induction. In addition, minocycline induced a down-regulation, predominantly, of M1 markers. Our results suggest early activation of M1 microglia that is followed by an overlap of both M1 and M2 phenotypes and that the beneficial effects of minocycline on sepsis-associated brain dysfunction may be related to its effects predominantly on the M1 phenotype.en
dc.format.mimetypeapplication/pdfpt_BR
dc.language.isoengpt_BR
dc.relation.ispartofJournal of cellular and molecular medicine. Oxford. Vol. 24, no. 1 (Jan. 2020), p. 88-97pt_BR
dc.rightsOpen Accessen
dc.subjectSepsept_BR
dc.subjectInflammationen
dc.subjectM1/M2en
dc.subjectFenótipopt_BR
dc.subjectMicrogliaen
dc.subjectMicrogliapt_BR
dc.subjectMicroglial polarizationen
dc.subjectHipocampopt_BR
dc.subjectPhenotypesen
dc.subjectSepsisen
dc.titleCharacterization and modulation of microglial phenotypes in an animal model of severe sepsispt_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb001114226pt_BR
dc.type.originEstrangeiropt_BR


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