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dc.contributor.authorFerreira, Carlos Alexandre Sanchezpt_BR
dc.contributor.authorBarbosa, M. C.pt_BR
dc.contributor.authorSilveira, Thiago Cesar Limapt_BR
dc.contributor.authorValenzuela, J.G.pt_BR
dc.contributor.authorVaz Junior, Itabajara da Silvapt_BR
dc.contributor.authorMasuda, Aoipt_BR
dc.date.accessioned2011-01-21T05:59:00Zpt_BR
dc.date.issued2002pt_BR
dc.identifier.issn0031-1820pt_BR
dc.identifier.urihttp://hdl.handle.net/10183/27454pt_BR
dc.description.abstractThe tick Boophilus microplus is a 1-host tick that causes important losses to bovine herds, and protective antigens are being investigated in order to develop vaccines that avoid the use of acaricides. Paramyosins are multi-functional invertebrate muscle proteins, whose roles may include host immunomodulation, and seem to be a prominent candidate in a schistosomiasis vaccine. We report here the cloning, expression and characterization of a B. microplus paramyosin (BmPRM). Sequence analysis of the full length coding sequence cDNA shows high identity to other arthropod paramyosin sequences, and the predicted molecular weight, pI and secondary structure are consistent with a typical paramyosin. Western-blot expression analysis indicates the presence of BmPRM in all tissues and developmental stages tested, but not in saliva. The recombinant protein (rBmPRM) was shown to bind both IgG and collagen. Possible implications of these activities with host evasion mechanisms are discussed.en
dc.format.mimetypeapplication/pdfpt_BR
dc.language.isoengpt_BR
dc.relation.ispartofParasitology. Inglaterra. Vol. 125, no. 3 (2002), p. 265-274pt_BR
dc.rightsOpen Accessen
dc.subjectParamyosinen
dc.subjectParasitologia veterináriapt_BR
dc.subjectBoophilus microplusen
dc.subjectIgG binding proteinen
dc.subjectCollagen binding proteinen
dc.subjectTicken
dc.titlecDNA cloning, expression and characterization of a Boophilus microplus paramyosin.pt_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb000337062pt_BR
dc.type.originEstrangeiropt_BR


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