High cholesterol diet exacerbates blood-brain barrier disruption in LDLr-/- mice : impact on cognitive function
dc.contributor.author | Oliveira, Jade de | pt_BR |
dc.contributor.author | Engel, Daiane Fátima | pt_BR |
dc.contributor.author | Paula, Gabriela Cristina de | pt_BR |
dc.contributor.author | Santos, Danúbia Bonfanti dos | pt_BR |
dc.contributor.author | Lopes, Jadna Bogado | pt_BR |
dc.contributor.author | Farina, Marcelo | pt_BR |
dc.contributor.author | Moreira, Eduardo Luiz Gasnhar | pt_BR |
dc.contributor.author | Bem, Andreza Fabro de | pt_BR |
dc.date.accessioned | 2024-10-19T06:18:12Z | pt_BR |
dc.date.issued | 2020 | pt_BR |
dc.identifier.issn | 1387-2877 | pt_BR |
dc.identifier.uri | http://hdl.handle.net/10183/280236 | pt_BR |
dc.description.abstract | Background: Evidence has revealed an association between familial hypercholesterolemia and cognitive impairment. In this regard, a connection between cognitive deficits and hippocampal blood-brain barrier (BBB) breakdown was found in low-density lipoprotein receptor knockout mice (LDLr–/–), a mouse model of familial hypercholesterolemia. Objective: Herein we investigated the impact of a hypercholesterolemic diet on cognition and BBB function in C57BL/6 wild-type and LDLr–/– mice. Methods: Animals were fed with normal or high cholesterol diets for 30 days. Thus, wild-type and LDLr–/– mice were submitted to memory paradigms. Additionally, BBB integrity was evaluated in the mice’s prefrontal cortices and hippocampi. Results: A tenfold elevation in plasma cholesterol levels of LDLr–/– mice was observed after a hypercholesterolemic diet, while in wild-type mice, the hypercholesterolemic diet exposure increased plasma cholesterol levels only moderately and did not induce cognitive impairment. LDLr–/– mice presented memory impairment regardless of the diet.We observed BBB disruption as an increased permeability to sodium fluorescein in the prefrontal cortices and hippocampi and a decrease on hippocampal claudin-5 and occludin mRNA levels in both wild-type and LDLr–/– mice treated with a hypercholesterolemic diet. The LDLr–/– mice fed with a regular diet already presented BBB dysfunction. The BBB-increased leakage in the hippocampi of LDLr–/– mice was related to high microvessel content and intense astrogliosis, which did not occur in the control mice. | en |
dc.format.mimetype | application/pdf | pt_BR |
dc.language.iso | eng | pt_BR |
dc.relation.ispartof | Journal of Alzheimer's disease. Amsterdam. Vol. 78, no. 1 (2020), p. 97-115 | pt_BR |
dc.rights | Open Access | en |
dc.subject | Blood-brain barrier | en |
dc.subject | Doenças neuroinflamatórias | pt_BR |
dc.subject | LDLr-/- mice | en |
dc.subject | Receptores de LDL | pt_BR |
dc.subject | Familial hypercholesterolemia | en |
dc.subject | Barreira hematoencefálica | pt_BR |
dc.subject | Memory impairment | en |
dc.subject | Disfunção cognitiva | pt_BR |
dc.subject | Amnésia | pt_BR |
dc.subject | Mild cognitive impairment | en |
dc.subject | Hiperlipoproteinemia Tipo II | pt_BR |
dc.subject | Neuroinflammation | en |
dc.title | High cholesterol diet exacerbates blood-brain barrier disruption in LDLr-/- mice : impact on cognitive function | pt_BR |
dc.type | Artigo de periódico | pt_BR |
dc.identifier.nrb | 001205540 | pt_BR |
dc.type.origin | Estrangeiro | pt_BR |
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