Mostrar registro simples

dc.contributor.authorSouza, Heryk Motta dept_BR
dc.contributor.authorReuwsaat, Júlia Catarina Vieirapt_BR
dc.contributor.authorLopes, Fernanda Cortezpt_BR
dc.contributor.authorViezzer, Gracielept_BR
dc.contributor.authorVolpato, Fabiana Caroline Zempulskipt_BR
dc.contributor.authorBarth, Afonso Luispt_BR
dc.contributor.authorDalcin, Paulo de Tarso Rothpt_BR
dc.contributor.authorStaats, Charley Christianpt_BR
dc.contributor.authorVainstein, Marilene Henningpt_BR
dc.contributor.authorSilva, Lívia Kmetzsch Rosa ept_BR
dc.date.accessioned2024-10-26T06:57:07Zpt_BR
dc.date.issued2024pt_BR
dc.identifier.issn1465-993Xpt_BR
dc.identifier.urihttp://hdl.handle.net/10183/280612pt_BR
dc.description.abstractBackground: Bronchiectasis is a condition characterized by abnormal and irreversible bronchial dilation resulting from lung tissue damage and can be categorized into two main groups: cystic fibrosis (CF) and non-CF bronchiectasis (NCFB). Both diseases are marked by recurrent infections, inflammatory exacerbations, and lung damage. Given that infections are the primary drivers of disease progression, characterization of the respiratory microbiome can shed light on compositional alterations and susceptibility to antimicrobial drugs in these cases compared to healthy individuals. Methods: To assess the microbiota in the two studied diseases, 35 subjects were recruited, comprising 10 NCFB and 13 CF patients and 12 healthy individuals. Nasopharyngeal swabs and induced sputum were collected, and total DNA was extracted. The DNA was then sequenced by the shotgun method and evaluated using the SqueezeMeta pipeline and R.pt_BR
dc.description.abstractResults: We observed reduced species diversity in both disease cohorts, along with distinct microbial compositions and profiles of antimicrobial resistance genes, compared to healthy individuals. The nasopharynx exhibited a consistent microbiota composition across all cohorts. Enrichment of members of the Burkholderiaceae family and an increased Firmicutes/Bacteroidetes ratio in the CF cohort emerged as key distinguishing factors compared to NCFB group. Staphylococcus aureus and Prevotella shahii also presented differential abundance in the CF and NCFB cohorts, respectively, in the lower respiratory tract. Considering antimicrobial resistance, a high number of genes related to antibiotic efflux were detected in both disease groups, which correlated with the patient’s clinical data. Conclusions: Bronchiectasis is associated with reduced microbial diversity and a shift in microbial and resistome composition compared to healthy subjects. Despite some similarities, CF and NCFB present significant differences in microbiome composition and antimicrobial resistance profiles, suggesting the need for customized management strategies for each disease.en
dc.format.mimetypeapplication/pdfpt_BR
dc.language.isoengpt_BR
dc.relation.ispartofRespiratory research. London. V. 25 (2024), e211, [18 p.]pt_BR
dc.rightsOpen Accessen
dc.subjectBronquiectasiapt_BR
dc.subjectAbnormal and irreversible bronchial dilationen
dc.subjectFibrose císticapt_BR
dc.subjectNon-CF bronchiectasisen
dc.subjectBronquiectasiapt_BR
dc.titleComparative microbiome analysis in cystic fbrosis and non-cystic fbrosis bronchiectasispt_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb001206667pt_BR
dc.type.originEstrangeiropt_BR


Thumbnail
   

Este item está licenciado na Creative Commons License

Mostrar registro simples