Pré-concentração de nitrosaminas a partir de amostras aquosas por extração em fase sólida e cromatografia capilar eletrocinética micelar

Abstract

This paper describes the development of a methodology for solid phase extraction (SPE) and pre-concentration of nitrosamines from aqueous samples using granular activated charcoal as stationary phase. Micelar Electrokinetic Capillary Chromatography (MEKC) was used for the separation and identification of the nitrosamines in the extracts. Using a sample with 50 µg L-1 of each nitrosamines standards (dimethylnitrosamine, DMN; diethylnitrosamine, DEN; N-nitrosopyrrolidine, NPYR; N-nitrosopiperidine, NPIP; Nnitrosomorpholine, NMOR), the methodology showed a range of recuperation from 29 to 107% with a linear zone between 10 and 500 µg L-1. The developed methodology can be applicable to the determination of these analytes in different aqueous samples.