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Minimum detection limit of an in-house nested-PCR assay for herpes simplex virus and varicella zoster virus

dc.contributor.authorRodrigues, Diógenespt_BR
dc.contributor.authorParis, Fernanda dept_BR
dc.contributor.authorPaiva, Rodrigo Minutopt_BR
dc.date.accessioned2014-05-06T02:04:56Zpt_BR
dc.date.issued2013pt_BR
dc.identifier.issn0037-8682pt_BR
dc.identifier.urihttp://hdl.handle.net/10183/94942pt_BR
dc.description.abstractHerpes simplex virus (H SV) and varicella zoster virus (VZV) are responsible for a variety of human diseases, including central nervous system diseases. The use of polymerase chain reaction (PCR) techniques on cerebrospinal fl uid samples has allowed the detection of viral DNA with high sensitivity and specifi city. Methods: Serial dilutions of quantifi ed commercial controls of each virus were subjected to an in-house nested-PCR technique. Results: The minimum detection limits for HSV and VZV were 5 and 10 copies/μL, respectively. Conclusions: The detection limit of nested-PCR for HSV and VZV in this study was similar to the limits found in previous studies.en
dc.format.mimetypeapplication/pdfpt_BR
dc.language.isoengpt_BR
dc.relation.ispartofRevista da Sociedade Brasileira de Medicina Tropical. Brasilia. Vol. 46, n. 5 (set./out. 2013), p. 625-628.pt_BR
dc.rightsOpen Accessen
dc.subjectPolymerase chain reactionenen
dc.subjectSimplexviruspt_BR
dc.subjectHerpesvirus Humano 3pt_BR
dc.subjectVaricella zoster virusen
dc.subjectHerpes simplex virusen
dc.subjectReação em cadeia da polimerasept_BR
dc.titleMinimum detection limit of an in-house nested-PCR assay for herpes simplex virus and varicella zoster viruspt_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb000906185pt_BR
dc.type.originNacionalpt_BR


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