Physiologic s100b concentrations reduces glucose uptake in c6 astroglial cells

Abstract

Astrocytes are the most common cells present in the brain and they are responsible for metabolic support, characterized mainly for their close interaction with neurons. Some glial markers are well recognized, as S100B, a calcium binding protein that plays important intra and extracellular roles in central nervous system. This protein is implicated in glial-neuron communication, presenting neurotrophic or neurotoxic properties, depending on its concentration. Among the models available to study astrocytes, C6 glioma cells in later than 100 passages are very useful, because have properties that are characteristic of the glial cells. The objective of the present work is to evaluate the effect of physiological concentration of exogenous S100B on glucose uptake in C6 astroglial cells. We observed a reduction of glucose uptake in the presence of exogenous S100B, and the incubation with anti-S100B antibody reversed this effect. The incubation with anti-RAGE antibody also reversed this effect, demonstrating that this receptor is involved in the S100B effect. However, further studies are necessary to unveil the S100B mechanism of action.