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dc.contributor.advisorBarth, Afonso Luispt_BR
dc.contributor.authorPancotto, Lisiane Rechpt_BR
dc.date.accessioned2017-05-17T02:36:46Zpt_BR
dc.date.issued2015pt_BR
dc.identifier.urihttp://hdl.handle.net/10183/158108pt_BR
dc.description.abstractCarbapenems are considered the last resort for the treatment of multidrug-resistant Enterobacteriaceae. The massive use of these agents, in addition to the horizontal transfer of resistance determinants, are responsible for the emergence and spread of carbapenemases. The rapid detection of these enzymes will allow early infection control measures, reducing the risk of therapeutic failure and avoiding the spread of the carbapenemases. The development of rapid tests for carbapenemase detection is mandatory. The aim of this study was to evaluate the performance of the rapid tests CARBA NP and BLUE-CARBA against carbapenemase-producing and non-carbapenemase-producing Enterobacteriaceae. A total of 90 isolates – 45 carbapenemase-producing- and 45 -non-producing-Enterobacteriaceae – were selected for this study. We performed both CARBA NP and BLUE-CARBA as previously described. We also added phenylboronic acid (PBA) in both assays to evaluate the presence of Class A carbapenemases. The sensitivity/specificity were 64.44%/97.78% and 91.11%/86.67% for CARBA NP and BLUE-CARBA, respectively. The modified assays with PBA did not provide any reliable results. Our results indicated that the BLUE-CARBA proved to be the best methodology (higher sensitivity) to be used as a screening test for carbapenemase detection.en
dc.format.mimetypeapplication/pdfpt_BR
dc.language.isoengpt_BR
dc.rightsOpen Accessen
dc.subjectFarmáciapt_BR
dc.subjectCarbapenemaseen
dc.subjectEnterobacteriaceaeen
dc.subjectKPCen
dc.subjectRapid testsen
dc.titleEvaluation of rapid phenotypic tests (CARBANP and BLUE-CARBA) for carbapenemase production among Enterobacteriaceaept_BR
dc.typeTrabalho de conclusão de graduaçãopt_BR
dc.contributor.advisor-coNodari, Carolina Silvapt_BR
dc.identifier.nrb001020388pt_BR
dc.degree.grantorUniversidade Federal do Rio Grande do Sulpt_BR
dc.degree.departmentFaculdade de Farmáciapt_BR
dc.degree.localPorto Alegre, BR-RSpt_BR
dc.degree.date2015pt_BR
dc.degree.graduationFarmáciapt_BR
dc.degree.levelgraduaçãopt_BR


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